A multimodal anion exchange resin designed for post-protein A purification of monoclonal antibodies at process scale. The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin. Capto adhere multimodal chromatography resin. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). The RNA fraction bound most strongly to the resin and could be eluted only. Wash with 5 CV of start buffer or until eluent pH and conductivity have reached the required values. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. The results showed that the Capto family had the highest critical velocities (Capto Adhere- 492, Capto Q- 477 cm/hr), whilst Sepharose CL-6B, Sepharose 4 Fast Flow and Sepharose CL-4B had the lowest critical velocity values (283, 204, 149 cm/hr respectively). Wash with at least 2 CV of 2 M NaCl at a pH > 9 (Capto MMC/Capto MMC ImpRes) or 0.5 M acetic acid (Capto adhere/Capto adhere ImpRes). This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. Capto Adhere proved to offer a very strong binding of nucleic acids. Capto Adhere proved to offer a very strong binding of nucleic acids. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Another pronounced difference between the resins was observed in the inverted elution of ss- and dsDNA, where ssDNA eluted at 2.88 M NaCl on Capto Adhere, while on Capto Q ImpRes ssDNA eluted already at 1.47 M NaCl. Capto adhere and especially Capto MMC thus present a truly different. Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. This recognition was not observed for Capto Adhere. Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin Methods Mol Biol. Some ion-exchange resins or the combination of resin and separation conditions may.
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